Laser Raman microscope RAMAN-11
RAMAN-11 is a state-of-art laser microscope designed for next generation microscopy. The Raman imaging method opens up non-stained imaging which enables in-vivo observation of living biological samples. Researchers can analyze constituent elements of organelles or proteins in detail with RAMAN-11. Neither harmful dyes nor unwanted staining procedure are needed any more.
Standard Raman microscopes were designed using a conventional scanning technique that scans the sample point by point. Due to the weakness of Raman scattering light, this scanning speed was very slow. It would take a few hours to image of living cells. RAMAN-11 has noteworthy capability to accelerate the imaging speed. A flying spot scanning technique contributes 100 times faster scanning than conventional method.
RAMAN-11 is equipped with a Differential Interference Contrast system and an epi-fluorescence system as well. A simple mouse click on the Raman image acquires the Raman spectrum easily. This function helps in interactive spectra analysis.Resolution in lateral direction: 500 nm Resolution in longitudinal direction: 1000 nm View field : 100 um x 140 um (at 100x objective)
Raman image of non-stained living cardiomyocytes. The cardiomyocytes were isolated from neonatal Whister rats and cultured for a few days. Raman spectral peaks at 1577cm-1, 1660cm-1, and 2935cm-1 indicate guanine/adenine molecules, Amide I, and skeletal vibration of C-H bonds respectively. In this image, the each peak is assigned on RGB color for display. This false color image reveals the organelles distribution. The distinct Raman spectra are observed in the image as color contrast.
Features
- Fast imaging capability of Raman spectrum
- Three dimensional resolution
- Eliminates fluorescent light by switching illumination wavelength.
- Raman spectrum displayed in detail by a simple mouse click.
>> Laser Raman microscope RAMAN-11 Specification
Main components
- Optical microscope (upright or inverted)
- Fast scanner
- High power laser (532nm and/or 785nm) for excitation.
- Spectrographs (f = 500mm, Czerny-Turner type)
- hermoelectrically cooled CCD detector (400 x 1340 pixels)
>> Specification (microscope) |
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Resolution in lateral direction |
500 nm |
Resolution in longitudinal direction |
lOOO nm |
View field |
lOOum X 140um (at 100x objective) |
Imaging speed for living cells |
60 s - (full spectrum detection over 2000 cm-1 whole viewing field.) |
Scanning system |
Flying spot illumination with servo Galvano mirrors. |
>> Specification (spectral) |
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Spectrum resolution |
1.6 cm-1(@785 nm excitation) |
Lower limit of wave number |
100 cm-1 |
Raman excitation wavelength |
532 / 785 nm switch-over |
Gratings |
Three gratings within motorized revolver |
>> Other equipment |
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>> Others |
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Dimensions |
720(W), 700(D), 800(H) |
Utility |
AC 90 - 120 V or 180 - 240V, 50 - 60Hz 7A |
Environment |
20-30 degree (non condensing) |